JoVE Journal
Cancer Research
Cancer Research
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Summary
Please note that all translations are automatically generated.
本协议描述了未转化的乳腺上皮细胞系MCF10A的3D “顶部”培养物的建立,该培养物已被修改以研究血小板活化因子(PAF)诱导的转化。免疫荧光已被用于评估转化,并详细讨论。