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Fosfolipidmediator induceret transformation i tredimensionelle kulturer
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures
DOI:

08:02 min

July 27, 2022

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Chapters

  • 00:04Introduction
  • 00:59Seeding MCF10A Cells in lrECM, PAF Treatment, and Re-Feeding with Fresh Media
  • 03:00Immunofluorescence Study to Detect Phenotypic Changes Induced by Prolonged PAF Exposure
  • 06:05Results: Nuclei of Cells in MCF10A Acini Stained with a Nuclear Stain
  • 07:25Conclusion

Summary

Automatic Translation

Den nuværende protokol beskriver oprettelsen af 3D 'ovenpå ' kulturer af en ikke-transformeret brystepitelcellelinje, MCF10A, der er blevet modificeret til at studere blodpladeaktiveringsfaktor (PAF) induceret transformation. Immunfluorescens er blevet brugt til at vurdere transformationen og diskuteres detaljeret.

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