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Journal
/
Developmental Biology
/
在蛋
和
卵外
研究中研究鸟类内耳发育的方法
JoVE Journal
Developmental Biology
Author Produced
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JoVE Journal
Developmental Biology
In Ovo
and
Ex Ovo
Methods to Study Avian Inner Ear Development
Please note that all translations are automatically generated.
Click here for the English version.
在蛋
和
卵外
研究中研究鸟类内耳发育的方法
DOI:
10.3791/64172-v
•
10:09 min
•
June 16, 2022
•
Nishant Singh*
1,2
,
Anubhav Prakash*
1
,
Suraj Ranganath Chakravarthy*
1
,
Raman Kaushik*
1
,
Raj K. Ladher
1
National Centre for Biological Sciences
,
Tata Institute of Fundamental Research
,
2
The University of Trans-Disciplinary Health Sciences and Technology
Chapters
00:05
Introduction
00:55
Egg Handling and Windowing
01:46
Microinjecting Plasmids
03:03
Electroporation
04:01
Basilar Papilla Dissection
06:04
Culture of Basilar Papilla Explants
07:41
Results: Molecular Mechanisms Underlying Avian Inner Ear Development and Regeneration
09:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
雏鸡是一种具有成本效益、可及且广泛可用的模式生物,可用于各种研究。在这里,详细介绍了一系列协议,以了解鸟类内耳发育和再生的分子机制。
Tags
Avian Inner Ear
Hair Cell Regeneration
Chick Embryo
Ototoxicity
Gene Knockout
Otic Vesicle
Electroporation
T7 Endonuclease Assay
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