Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection

Matloob Husain

doi:10.3791/64189

Journal

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Immunology and Infection

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Identifiera kaspaser och deras motiv som klyver proteiner under influensa A-virusinfektion

JoVE Journal
Immunology and Infection
Author Produced

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JoVE Journal Immunology and Infection

Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection

Please note that all translations are automatically generated. Click here for the English version.

Identifiera kaspaser och deras motiv som klyver proteiner under influensa A-virusinfektion

DOI:

10.3791/64189-v

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08:16 min

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July 21, 2022

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Matloob Husain

¹Department of Microbiology and Immunology,University of Otago

Chapters

00:04Introduction
00:45Assessing the Cleavage of Proteins by Caspases in IAV-Infected Cells
02:55Confirmation of Caspase-Mediated Cleavage of Proteins in IAV-Infected Cells by RNA Interference
04:13Site-Directed Mutagenesis for Locating the Caspase Cleavage Sites in the Polypeptide
05:49Results: Role of Caspases in IAV Infection-Induced Degradation of Cortactin
07:32Conclusion

Summary

Automatic Translation

Influensa A-virus (IAV) -infektion aktiverar de caspaser som klyver värd- och virusproteiner, som i sin tur har pro- och antivirala funktioner. Genom att använda hämmare, RNA-interferens, platsriktad mutagenes och western blotting- och RT-qPCR-tekniker identifierades kaspaser i infekterade däggdjursceller som klyver värdkortaktin och histondeacetylaser.