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JoVE Journal Genetics
C. elegans Gonaddissektion og fryse revne til immunfluorescens og DAPI-farvning
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C. elegans Gonaddissektion og fryse revne til immunfluorescens og DAPI-farvning

Article DOI: 10.3791/64204-v • 06:04 min • September 16th, 2022
September 16th, 2022 •
*1, *1, 1, 1
1Department of Biological Sciences, University of Massachusetts Lowell
* These authors contributed equally

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Dette er en almindeligt anvendt metode til C. elegans gonaddissektion efterfulgt af frysecrack, som producerer kimlinjeprøver til immunfluorescens via antistoffarvning eller til simpel DAPI-farvning for at visualisere DNA. Denne protokol har været vellykket for studerende i et forskningslaboratorium og i en kursusbaseret bachelorforskningserfaring.

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Genetik udgave 187
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