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Journal / Developmental Biology / Fluoreszierende In situ Hybridisierung und…
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JoVE Journal Developmental Biology
Fluoreszierende In situ Hybridisierung und 5-Ethynyl-2'-Desoxyuridin-Markierung für stammähnliche Zellen in der Hydrozoenqualle Cladonema pacificum
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Fluoreszierende In situ Hybridisierung und 5-Ethynyl-2'-Desoxyuridin-Markierung für stammähnliche Zellen in der Hydrozoenqualle Cladonema pacificum

Article DOI: 10.3791/64285-v • 08:44 min • August 3rd, 2022
August 3rd, 2022 •
1, 1, 2, 2
1Graduate School of Life Sciences, Tohoku University, 2Graduate School of Pharmaceutical Sciences, The University of Tokyo

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Hier beschreiben wir ein Protokoll zur Visualisierung stammartig wuchernder Zellen in der Qualle Cladonema. Die Whole-mount-Fluoreszenz-in-situ-Hybridisierung mit einem Stammzellmarker ermöglicht den Nachweis stammähnlicher Zellen, und die 5-Ethynyl-2'-Desoxyuridin-Markierung ermöglicht die Identifizierung proliferierender Zellen. Zusammen können aktiv wuchernde stammartige Zellen nachgewiesen werden.

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Developmental Biology Ausgabe 186 FISH EdU Quallen Medusa Cladonema pacificum Stammzellen Zellproliferation
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