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体外纳米乳剂疫苗佐剂蛇腓磷素D的细胞活性评价

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Immunology and Infection

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JoVE Journal Immunology and Infection

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

Please note that all translations are automatically generated. Click here for the English version.

体外纳米乳剂疫苗佐剂蛇腓磷素D的细胞活性评价

DOI:

10.3791/64291-v

•

08:15 min

•

December 09, 2022

•

Xing Luo, Yanan Tong, Xiaoqiang Zeng, Yan Ye, Yun Yang, Zhen Song, Zelong Zhang, Haibo Li, Jining Gao, Xuhu Mao, Hao Zeng, Quanming Zou, Hongwu Sun

¹Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy,Third Military Medical University of Chinese PLA, ²Institute of Combined Injury of PLA, College of Military Preventive Medicine,Third Military Medical University of Chinese PLA

Chapters

00:05Introduction
00:55L929 Cytotoxicity Assay
01:51Splenocyte Stimulation
04:02Uptake by DCs
06:09Macrophage Activation
06:57Results: Impact of Adjuvant-Formulated Antigen on the Activation of PMs
07:52Conclusion

Summary

Automatic Translation

该协议提供了评估纳米乳剂蛇腓苷D佐剂是否促进有效细胞免疫应答的详细方法。

Tags

Keywords: In Vitro Cellular Activity Nanoemulsion Vaccine Adjuvant Ophiopogonin D Cellular Response Adjuvant Evaluation L929 Cells DMEM Centrifugation Mouse Spleen Red Blood Cell Lysis Cellular Delivery

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