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Journal
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Immunology and Infection
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インビトロ
ナノエマルジョンワクチンアジュバントオフィオポゴニンDの細胞活性評価
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
In Vitro
Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
Please note that all translations are automatically generated.
Click here for the English version.
インビトロ
ナノエマルジョンワクチンアジュバントオフィオポゴニンDの細胞活性評価
DOI:
10.3791/64291-v
•
08:15 min
•
December 09, 2022
•
Xing Luo
,
Yanan Tong
,
Xiaoqiang Zeng
,
Yan Ye
,
Yun Yang
,
Zhen Song
,
Zelong Zhang
,
Haibo Li
,
Jining Gao
,
Xuhu Mao
,
Hao Zeng
,
Quanming Zou
,
Hongwu Sun
1
Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy
,
Third Military Medical University of Chinese PLA
,
2
Institute of Combined Injury of PLA, College of Military Preventive Medicine
,
Third Military Medical University of Chinese PLA
Chapters
00:05
Introduction
00:55
L929 Cytotoxicity Assay
01:51
Splenocyte Stimulation
04:02
Uptake by DCs
06:09
Macrophage Activation
06:57
Results: Impact of Adjuvant-Formulated Antigen on the Activation of PMs
07:52
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、ナノエマルジョンオフィオポゴニンDアジュバントが効果的な細胞性免疫応答を促進するかどうかを評価するための詳細な方法を提示する。
Tags
In Vitro
Cellular Activity
Nanoemulsion
Vaccine Adjuvant
Ophiopogonin D
Cellular Response
Adjuvant Evaluation
L929 Cells
DMEM
Centrifugation
Mouse Spleen
Red Blood Cell Lysis
Cellular Delivery
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