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Journal
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Immunology and Infection
/
大鼠烧伤模型研究全层皮肤热烧伤和感染
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Rat Burn Model to Study Full-Thickness Cutaneous Thermal Burn and Infection
Please note that all translations are automatically generated.
Click here for the English version.
大鼠烧伤模型研究全层皮肤热烧伤和感染
DOI:
10.3791/64345-v
•
08:40 min
•
August 23, 2022
•
Rajnikant Sharma
,
Shekhar Yeshwante
,
Quentin Vallé
,
Maytham Hussein
,
Varsha Thombare
,
Sean Michael McCann
,
Robert Maile
4,5
,
Jian Li
,
Tony Velkov
,
Gauri Rao
1
UNC Eshelman School of Pharmacy
,
University of North Carolina at Chapel Hill
,
2
Department of Biochemistry & Pharmacology, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences
,
The University of Melbourne
,
3
Department of Microbiology & Immunology
,
University of North Carolina School of Medicine
,
4
Department of Surgery
,
University of North Carolina at Chapel Hill
,
5
Curriculum in Toxicology and Environmental Medicine
,
University of North Carolina at Chapel Hill
,
6
Department of Microbiology, Monash Biomedicine Discovery Institute
,
Monash University
Chapters
00:05
Introduction
00:57
Preparing Rats for the Burn Injury
01:54
Inducing the Burn Injury in Rats
03:20
Preparation of Bacterial Inoculum and Infection
04:26
Evaluation of the Burn Injury and Postprocessing of Rat Samples and Bacterial Enumeration
05:37
Results: A Simple and Reproducible Rat Burn Infection Model
07:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
模拟烧伤和感染临床情况的模型对于进一步烧伤研究是必要的。本协议展示了一种与人类相当的简单且可重复的大鼠烧伤感染模型。这有助于研究烧伤后烧伤和感染,以开发新的局部抗生素治疗。
Tags
Keywords: Rat Burn Model
Full-thickness Cutaneous Thermal Burn
Bacterial Infection
Wound Healing
Thermal Burn Induction
Burn Injury
Burn Wound
Pseudomonas Aeruginosa
Staphylococcus Aureus
Animal Model
Copper Rod
Isoflurane
Morphine
Lactated Ringer’s Solution
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