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秀丽隐杆线虫种系内突触复合体的超分辨率显微镜
JoVE Journal
Biology
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JoVE Journal Biology
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
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秀丽隐杆线虫种系内突触复合体的超分辨率显微镜

DOI:
10.3791/64363-v

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09:14 min

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September 13, 2022

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, , , ,
1Cell Biology and Biophysics,European Molecular Biology Laboratory, 2Collaboration for joint PhD degree between EMBL and Heidelberg University,Faculty of Biosciences, 3EMBL Imaging Centre,European Molecular Biology Laboratory

Chapters

  • 00:04Introduction
  • 01:04Dissection and Fixation
  • 02:52Incubation with Antibodies
  • 04:00Mounting Samples on a Coverslip
  • 05:27Imaging
  • 07:30Results: Visualization of Synaptonemal Complex Using Super‐Resolution Microscopy
  • 08:34Conclusion

Summary

Automatic Translation

Automatic Translation

超分辨率显微镜可以详细了解减数分裂中突触复合物内成分的组织。在这里，我们展示了一种解析 秀丽隐杆线虫 突触复合物的单个蛋白质的方案。

Tags

Super-resolution MicroscopySynaptonemal ComplexCaenorhabditis ElegansGermlineMeiosisImmunostainingSingle Molecule Localization MicroscopyDissectionFixationAntibody Staining

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