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Et opløseligt tetrazoliumbaseret reduktionsassay for at evaluere virkningen af antistoffer på Candida tropicalis biofilm
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

Et opløseligt tetrazoliumbaseret reduktionsassay for at evaluere virkningen af antistoffer på Candida tropicalis biofilm

DOI:
10.3791/64425-v

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06:50 min

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September 16, 2022

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, ,
1Department of Biosciences and Bioengineering,Indian Institute of Technology Roorkee

Chapters

  • 00:04Introduction
  • 01:26C. tropicalis Biofilm Formation
  • 02:40Treatment of Biofilm with Antibodies
  • 03:30Biofilm Metabolic Activity Estimation
  • 05:14Results: Effectiveness of Sap2-Specific Polyclonal Antibodies Against Preformed C. tropicalis Biofilms
  • 06:04Conclusion

Summary

Automatic Translation

Automatic Translation

Et 96-brønds mikrotiterpladebaseret protokol ved anvendelse af en 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-carboxanilid-2H-tetrazolium (XTT) reduktionsassay er beskrevet heri for at studere antistoffers virkninger på biofilm dannet af C. tropicalis. Denne in vitro-protokol kan bruges til at kontrollere virkningen af potentielle nye svampedræbende forbindelser på den metaboliske aktivitet af Candida-artsceller i biofilm.

Tags

BiofilmCandida TropicalisAntibodyAntifungalMetabolic ActivityTetrazolium-based Reduction Assay96-well PlateRPMI 1640 MOPS MediumPBS WashSerum DilutionNo-fungus Negative Control

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