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Developmental Biology

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JoVE Journal Developmental Biology
Ablação a Laser Multifóton de Centros Organizadores de Microtúbulos Citoplasmáticos em Oócitos de Camundongos
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Ablação a Laser Multifóton de Centros Organizadores de Microtúbulos Citoplasmáticos em Oócitos de Camundongos

Article DOI: 10.3791/64439-v • 08:24 min • November 11th, 2022
November 11th, 2022 •
1,2, 3, 1
1Animal Sciences Research Center, University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core, University of Missouri

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Apresenta-se um protocolo otimizado que permite a depleção de centros organizadores de microtúbulos citoplasmáticos em ovócitos de camundongos durante a metáfase I utilizando um laser de femtossegundos no infravermelho próximo.

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Biologia do Desenvolvimento Edição 189 Oócito MTOC citoplasmático metafásico meiose ablação a laser
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