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Journal
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Neuroscience
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亚细胞分离用于从小鼠脑中分离突触成分
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
Please note that all translations are automatically generated.
Click here for the English version.
亚细胞分离用于从小鼠脑中分离突触成分
DOI:
10.3791/64574-v
•
12:14 min
•
September 14, 2022
•
Sofia Massaro Tieze
2,3
,
Sreeganga S. Chandra
2
,
D. J. Vidyadhara
2
1
Department of Neurology
,
Yale University
,
2
Department of Neuroscience
,
Yale University
,
3
Interdepartmental Neuroscience Program
,
Yale University
Chapters
00:04
Introduction
01:37
Mouse Brain Excision
03:32
Synaptosome Preparation
05:23
Hypotonic Lysis
06:24
Synaptic Vesicle Isolation
07:35
Synaptic Plasma Membrane Isolation
10:02
Results: Electron Microscopy of Synaptosomes and Immunoblot Analysis of Subcellular Fractions
11:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议提供了一种强大,详细的方法,可以从小鼠大脑中获得高纯度的突触体,突触囊泡和其他突触部分。该方法能够评估突触过程,包括蛋白质定位和功能(具有区室分辨率)的生化分析。
Tags
Subcellular Fractionation
Synapses
Synaptosome
Brain Disorders
Protein Distribution
Protein Activity
Buffer Preparation
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