Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

This content is Open Access.

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
 

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Article DOI: 10.3791/61611-v
July 30th, 2020

Chapters

Summary July 30th, 2020

The activation of the multifunctional Ser/Thr kinase AURKA is hallmarked by its autophosphorylation on Thr288. Fluorescent probes relying on FRET can discriminate between its inactive and activated states. Here, we illustrate some strategies for probe engineering, together with a rapid FRET protocol to follow the kinase activation throughout mitosis.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter