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JoVE Journal Bioengineering
Hurtig udvikling af celletilstandsidentifikationskredsløb med polytransfektion
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Hurtig udvikling af celletilstandsidentifikationskredsløb med polytransfektion

Article DOI: 10.3791/64793-v • 09:21 min • February 24th, 2023
February 24th, 2023 •
1, 1,2, 3, 1,3
1Department of Biological Engineering, Massachusetts Institute of Technology, 2School of Biological Engineering, University of British Columbia, 3Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology

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Komplekse genetiske kredsløb er tidskrævende at designe, teste og optimere. For at lette denne proces transfekteres pattedyrceller på en måde, der tillader test af flere støkiometrier af kredsløbskomponenter i en enkelt brønd. Denne protokol skitserer trinene til eksperimentel planlægning, transfektion og dataanalyse.

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Bioengineering udgave 192 syntetisk biologi genetiske kredsløb celleklassifikator optimering af høj kapacitet hurtig prototyping af genetisk kredsløb transfektion flowcytometrianalyse
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