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Procedure for udvikling af multi-dybde cirkulære Tværsnit Endothelialized Microchannels-on-a-chip
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Chapters
- 00:05Title
- 02:22Photolithography Fabrication of Photoresist Master Mold
- 03:10Soft Lithography Fabrication of PDMS Microchannel Network
- 04:34HUVEC Culture and Seeding in the Chip
- 07:03Cell Staining and Microscope Characterization
- 08:10Results: Multi-depth Microchannel Networks Mimic the Complex 3D Geometries of In vivo Microvessel
- 09:35Conclusion
En microchannels-on-a-chip platform blev udviklet af en kombination af fotolitografisk reflowable photoresist teknik, blød litografi og mikrofluidik. Den endothelialized microchannels platform efterligner den tredimensionelle (3D) geometri in vivo mikrokar kører under kontrollerede kontinuerlig perfusion flow, giver mulighed for høj kvalitet og real-time scanning, og kan anvendes til microvascular forskning.
