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Rapid Development of Cell State Identification Circuits with Poly-Transfection
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Bioengineering
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JoVE Journal Bioengineering
Rapid Development of Cell State Identification Circuits with Poly-Transfection

Rapid Development of Cell State Identification Circuits with Poly-Transfection

DOI:
10.3791/64793-v

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09:21 min

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February 24, 2023

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, , ,
1Department of Biological Engineering,Massachusetts Institute of Technology, 2School of Biological Engineering,University of British Columbia, 3Department of Electrical Engineering and Computer Science,Massachusetts Institute of Technology

Chapters

  • 00:04Introduction
  • 00:43Transfection and Flow Cytometry
  • 04:59Results: Poly-Transfection: A High-Throughput Extension of Traditional Mammalian Transfection
  • 08:31Conclusion

Summary

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Complex genetic circuits are time-consuming to design, test, and optimize. To facilitate this process, mammalian cells are transfected in a way that allows the testing of multiple stoichiometries of circuit components in a single well. This protocol outlines the steps for experimental planning, transfection, and data analysis.

Tags

Keywords: Cell State IdentificationPoly-transfectionSynthetic BiologyFlow CytometryReporter GenesTransfectionTransfection ReagentPlasmidFiller PlasmidReduced Serum MediumEnhancer ReagentMKO2TagBFPNeon GreenL7Ae

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