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Journal / Developmental Biology / AMEBaS: Automatisk midterlinjeekstraktion og…
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Developmental Biology
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Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Journal (Developmental Biology)
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens
Journal (Developmental Biology)
3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens
 
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AMEBaS: Automatisk midterlinjeekstraktion og baggrundssubtraktion af ratiometrisk fluorescenstidsforløb af polariserede enkeltceller

Article DOI: 10.3791/64857-v • 06:03 min • June 23rd, 2023
June 23rd, 2023 •
1, 1,2, 3, 4, 5, 6, 1, 1
1Institute of Mathematics and Statistics, University of São Paulo, 2Center for Mathematics, Computing and Cognition, Federal University of ABC, 3Department of Botany, Institute of Biosciences, University of São Paulo, 4Cell Biology and Molecular Genetics Department, University of Maryland, 5Department of Biosciences, University of Milan, 6Department of Physics, Politecnico di Milano

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Nuværende metoder til analyse af polariserede enkeltcellers intracellulære dynamik er ofte manuelle og mangler standardisering. Dette manuskript introducerer en ny billedanalysepipeline til automatisering af midterlinjeekstraktion af enkeltpolariserede celler og kvantificering af rumlig tidsmæssig adfærd fra tidsforløb i en brugervenlig onlinegrænseflade.

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Udviklingsbiologi udgave 196 Baggrundssubtraktion Ratiometrisk fluorescens time-lapses polariserede enkeltceller cellepolaritet makroskopisk fænomen rumligt koncentrerede molekyler specialiserede domæner subcellulært niveau asymmetriske morfologiske strukturer celledeling vækst migration forstyrrelse af cellepolaritet vævsrelaterede lidelser kræft gastrisk dysplasi spatiotemporal dynamik fluorescerende reportere manuelle trin midterlinjesporing tidskrævende bias ratiometrisk Analyse ujævn fordeling af reportermolekyler baggrundssubtraktionsteknikker beregningsrørledning automatiser og kvantificer spatiotemporal adfærd enkeltceller pollenrør / rodhårvækst cytosolisk iondynamik
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