Journal
/
Neuroscience
/
Subcellular Imaging of Neuronal Calcium Handling In Vivo
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Subcellular Imaging of Neuronal Calcium Handling In Vivo

Subcellular Imaging of Neuronal Calcium Handling In Vivo

DOI:
10.3791/64928-v

•

07:14 min

•

March 17, 2023

•

, , ,
1Department of Biomedical Sciences,Colorado State University College of Veterinary Medicine and Biomedical Sciences, 2Cellular and Molecular Biology Graduate Program,Colorado State University College of Veterinary Medicine and Biomedical Sciences

Chapters

  • 00:04Introduction
  • 00:51Creating Transgenic Strains
  • 01:47Optical Setup
  • 02:21Preparation of Worms for Imaging
  • 04:14Acquisition of High‐Resolution Image Streams
  • 04:52Results: Rapid Imaging of Calcium Uptake in AVA Neurite
  • 06:19Conclusion

Summary

Automatic Translation

Automatic Translation

The current methods describe a non-ratiometric approach for high-resolution, sub-compartmental calcium imaging in vivo in Caenorhabditis elegans using readily available genetically encoded calcium indicators.

Tags

Subcellular ImagingNeuronal Calcium HandlingIn VivoC. ElegansCalcium IndicatorTransgenic StrainsTime-lapse ImagingAgar PadsFluorescent ToolsCalcium SignalingNeurodegeneration

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

In vivo Neuronal Calcium Imaging in C. elegans

Super-resolution Imaging of Neuronal Dense-core Vesicles

In Vivo Imaging of Dauer-specific Neuronal Remodeling in C. elegans

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish

In Vivo Optical Calcium Imaging of Learning-Induced Synaptic Plasticity in Drosophila melanogaster

In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity

In vivo Calcium Imaging in Mouse Inferior Olive

Read Article