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On-Section Correlative Light-Electron Microscopy를 통한 내인성 LC3의 초구조적 국소화
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On-Section Correlative Light-Electron Microscopy를 통한 내인성 LC3의 초구조적 국소화

Article DOI: 10.3791/65067-v • 11:53 min • March 31st, 2023
March 31st, 2023 •
1, 1, 1, 1, 1, 1, 1
1Center for Molecular Medicine-Cell Biology, University Medical Center Utrecht, Utrecht University

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여기서는 세포 초미세 구조와 관련된 희귀 단백질의 국소화를 조사하기 위한 도구로서 내인성 형광 표지를 기반으로 하는 최적화된 절편 상관 광전자 현미경을 위한 프로토콜을 제시합니다. 이 접근법의 힘은 바필로마이신 처리 없이 굶주린 세포에서 내인성 LC3의 초구조적 국소화에 의해 입증되었습니다.

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생물학 Correlative Light-electron Microscopy Autophagic Organelles Electron Microscopy Fluorescence Microscopy Autophagy Proteins Autophagosomes On-section CLEM LC3 Labeling Lysosomal Acidification Autolysosomes
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