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JoVE Journal
Biology

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内源性LC3的超微结构定位-截面相关光电子显微镜
 
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内源性LC3的超微结构定位-截面相关光电子显微镜

Article DOI: 10.3791/65067-v 11:53 min March 31st, 2023
March 31st, 2023

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Summary

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Click here for the English version.

在这里,我们提出了一种基于内源性荧光标记的优化截面相关光电子显微镜的方案,作为研究稀有蛋白质与细胞超微结构相关的定位的工具。这种方法的力量通过内源性LC3在饥饿细胞中的超微结构定位而得到证明,而没有巴弗洛霉素处理。

Tags

生物学,第 193 期,相关光电子显微镜,自噬细胞器,电子显微镜,荧光显微镜,自噬蛋白,自噬体,截面 CLEM,LC3 标记,溶酶体酸化,自溶酶体
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