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JoVE Journal Developmental Biology
Forberedelse av 3D decellulariserte matriser fra musens skjelettmus for cellekultur
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Forberedelse av 3D decellulariserte matriser fra musens skjelettmus for cellekultur

Article DOI: 10.3791/65069-v • 07:44 min • March 3rd, 2023
March 3rd, 2023 •
1, 1, 1, 1
1Centre for Ecology, Evolution and Environmental Changes and Global Change & Sustainability Institute, Department of Animal Biology, Faculty of Sciences, University of Lisbon

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I dette arbeidet ble en decellulariseringsprotokoll optimalisert for å oppnå decellulariserte matriser av fostermusens skjelettmus. C2C12 myoblaster kan kolonisere disse matrisene, spre seg og differensiere. Denne in vitro-modellen kan brukes til å studere celleadferd i sammenheng med skjelettmuskelsykdommer som muskeldystrofi.

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