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Chapters
- 00:05Title
- 02:01Initial Surgery
- 03:49Isolate the Innervation of the Medial Gastrocnemius Muscle and Achilles Tendon
- 05:25Cutting the Tibia and Mounting in the Apparatus
- 07:22Stimulating the Muscle
- 08:33Force-frequency Relationship
- 09:08Conclusion
This video demonstrates the surgical preparation and procedures needed to study the contractile responses of the rat medial gastrocnemius muscle preparation in situ. This preparation allows measurement of skeletal muscle contractile properties under physiological conditions. The animal is anesthetized and the muscle is separated from surrounding tissue at its distal end. The Achilles tendon is attached to a force transducer, allowing measurement of the muscle’s contractile response at 37 degrees C with an intact circulation.
Tags
In Situ Skeletal Muscle Contractile PropertiesRat Muscle PreparationPhysiological CircumstancesPosttetanic PotentiationStaircaseFatigueDiseaseDisuseInjuryTrainingDrug TreatmentAnesthesiaMedial Gastrocnemius Muscle IsolationBlood SupplyNerve SupplySciatic Nerve ClearanceStainless Steel Stimulating WiresCalcaneus SeveranceSonometric CrystalsElectromyography ElectrodesImmobilizationForce TransducerParaffin Oil
