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孤立大鼠心脏长期心室颤动模型
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JoVE Journal Medicine
A Model of Long-Term Ventricular Fibrillation in Isolated Rat Hearts
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孤立大鼠心脏长期心室颤动模型

DOI:
10.3791/65101-v

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07:56 min

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February 17, 2023

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, , ,
1Cardiovascular Surgery, First Medical Center,Chinese PLA General Hospital, 2Medical School of Chinese PLA

Chapters

  • 00:04Introduction
  • 00:53Preparing the Langendorff Apparatus
  • 02:01Preparing the Hardware and Software
  • 02:53Preparing the Isolated Heart
  • 04:20Perfusing and Electrically Stimulating the Heart
  • 05:34Results: Success Rate of Long‐Term Ventricular Fibrillation
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

该协议提出了由低压交流电连续刺激引起的大鼠心脏长期心室颤动的模型。该模型成功率高，稳定可靠，可重复，对心脏功能影响小，仅引起轻度心肌损伤。

Tags

Keywords: Ventricular FibrillationRat ModelLangendorff ApparatusKrebs-Henseleit BufferPerfusionECGElectrical StimulationAnesthesiaDissectionCardiac Surgery

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