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Focused Ultrasound Neuromodulation of Human In Vitro Neural Cultures in Multi-Well Microelectrode Arrays
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Focused Ultrasound Neuromodulation of Human In Vitro Neural Cultures in Multi-Well Microelectrode Arrays

Focused Ultrasound Neuromodulation of Human In Vitro Neural Cultures in Multi-Well Microelectrode Arrays

DOI:
10.3791/65115-v

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04:00 min

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May 03, 2024

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, , , , , , , , ,
1Electrical and Computer Engineering,Johns Hopkins University, 2HEPIUS Innovation Laboratory,Johns Hopkins University School of Medicine, 3Neurosurgery,Johns Hopkins University School of Medicine, 4Biomedical Engineering,Johns Hopkins University School of Medicine, 5Mechanical Engineering,Johns Hopkins University, 6Anesthesiology and Critical Care Medicine,Johns Hopkins University School of Medicine

Chapters

  • 00:00Introduction
  • 00:37Preparation of Materials and Setting Up the Experiment
  • 01:23Stimulation and Neuronal Signal Acquisition
  • 02:18Results: The Neuromodulatory Effects of Focused Ultrasound (FUS) on Neurons
  • 03:07Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for using a high-throughput system that enables the monitoring and quantification of the neuromodulatory effects of focused ultrasound on human-induced pluripotent stem cell (HiPSC) neurons.

Tags

Keywords: Focused UltrasoundNeuromodulationNeural CulturesMicroelectrode ArraysHIPSCsNeuronal ActivityFiring RateFUS ParametersIn Vitro Testing

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