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Neural Explant Cultures from Xenopus laevis
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Neural Explant Cultures from Xenopus laevis
DOI:

10:05 min

October 15, 2012

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Chapters

  • 00:05Title
  • 00:57Introduction
  • 01:14In vitro Fertilization of Xenopus Eggs
  • 02:34Microinjection of RNA
  • 04:46Neural Tube Dissection and Plating
  • 09:08Results: Imaging Growth Cones from Xenopus Neuronal Explants
  • 09:40Conclusion

Summary

Automatic Translation

Culturing neural explants from dissected Xenopus laevis embryos that express fluorescent fusion proteins allows for imaging of growth cone cytoskeletal dynamics.

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