JoVE Journal
Biology
Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Please note that all translations are automatically generated.
In this video, we are showing how to label human embryonic stem cells (hESC) with manganese chloride (MnCl2) which can enter cells via voltage-gated calcium channels when the cells are biologically active. Additionally, we show the use of MnCl2 as cellular MRI contrast agent to determine the in vitro viability of hESC.