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Journal / Immunology and Infection / Isolation of Mouse Peritoneal Cavity Cells
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Mouse Peritoneal Cavity Cells
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Isolation of Mouse Peritoneal Cavity Cells

Article DOI: 10.3791/1488-v • 04:32 min • January 28th, 2010
January 28th, 2010 •
1, 1
1BloodCenter of Wisconsin, Blood Research Institute

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The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.

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Peritoneal Cavity Cells Isolation Immune Cells Macrophages B Cells T Cells Naïve Macrophages Tissue Resident Macrophages Peritoneal Cavity-resident B Cell Subset B1 Cells B1a Cells B1b Cells CD11b CD5 Autoreactivity Autoimmunity IL-10 Producing Capacity
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