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Measurement of γHV68 Infection in Mice
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measurement of γHV68 Infection in Mice
DOI:

13:22 min

November 22, 2011

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Chapters

  • 00:05Title
  • 01:26Intranasal Infection of Mice with γHV68
  • 02:26Plaque Assay: Viral Titer Determination in Acutely Infected Lungs
  • 06:50Infectious Center Assay: Virus Load in Latently Infected Splenocytes
  • 10:47Representative Assay Results
  • 13:01Conclusion

Summary

Automatic Translation

γ-Herpesviruses (γ-HVs) establish life-long persistency in their host. Infection of mice with γ-HV68 provides a genetically tractable in vivo model for the characterization of the lifecycle/pathogenesis of γHVs. This protocol describes the detection and quantitation of γHV68 infection at acute and latent stages following infection by plaque-forming, infectious center, and qPCR assays.

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