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JoVE Journal Immunology and Infection
Primær microglia Isolasjon fra Blandede glial cellekulturer fra Neonatal Rat hjernevev
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Primær microglia Isolasjon fra Blandede glial cellekulturer fra Neonatal Rat hjernevev

Article DOI: 10.3791/3814-v • 10:20 min • August 15th, 2012
August 15th, 2012 •
1, 1,2,3, 2
1Neuroscience Program, Uniformed Services University, 2Department of Anatomy, Physiology, and Genetics, Uniformed Services University, 3Molecular and Cell Biology, Uniformed Services University

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Isolere primære microglia fra mobilnettet heterogenitet av hjernen er viktig å undersøke deres rolle i både fysiologiske og patologiske forhold. Denne protokollen beskriver en mekanisk isolering og blandet cellekultur teknikk som gir høy avkastning og høy renhet, levedyktige primære microglial celler for

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Immunologi Neuroscience fysiologi molekylærbiologi cellekultur isolasjon microglia blandet glial celle traumatisk hjerneskade nevrodegenerativ sykdom
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