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Immunology and Infection

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初级小胶质细胞的分离，从新生大鼠脑组织胶质细胞混合文化

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Immunology and Infection

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JoVE Journal Immunology and Infection

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Please note that all translations are automatically generated. Click here for the English version.

初级小胶质细胞的分离，从新生大鼠脑组织胶质细胞混合文化

DOI:

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10:20 min

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August 15, 2012

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Tami T. Tamashiro, Clifton Lee Dalgard^2,3, Kimberly R. Byrnes

¹Neuroscience Program,Uniformed Services University, ²Department of Anatomy, Physiology, and Genetics,Uniformed Services University, ³Molecular and Cell Biology,Uniformed Services University

Chapters

00:05Title
02:05Dissection of Neonatal Brain Tissue
03:28Preparation of Mixed Glial Cell Population
04:36Plating and Maintenance of Mixed Glial Cell Cultures
05:36Isolation and Plating of Primary Microglia
06:50Results: Cell-type Specific Immunostaining and Response of Microglial Cultures to Lipopolysaccharide
08:46Conclusion

Summary

Automatic Translation

隔离初级小胶质细胞的大脑细胞的异质性是必不可少的，以调查他们在生理和病理条件下的作用。本协议描述了机械隔离和混合细胞培养技术，提供高产量和高纯度的，可行的初级小胶质细胞<em>在体外</em>学习和下游应用。

Tags

Primary Microglia Isolation Mixed Glial Cell Cultures Microglia Role In Brain Physiology Phagocytic Activity Microglial Activation Neurodegenerative Disease Traumatic Brain Injury Nervous System Infection Reactive Oxygen And Nitrogen Species Pro-inflammatory Chemokines And Cytokines Cellular Heterogeneity Of The Brain Neuroprotective And Neurotoxic Functions Of Microglia Isolation Protocol For Primary Microglia

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