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Primære Mikroglia Isolering af blandede gliacelle Kulturer af neonatal rottehjerne Tissue
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Chapters
- 00:05Title
- 02:05Dissection of Neonatal Brain Tissue
- 03:28Preparation of Mixed Glial Cell Population
- 04:36Plating and Maintenance of Mixed Glial Cell Cultures
- 05:36Isolation and Plating of Primary Microglia
- 06:50Results: Cell-type Specific Immunostaining and Response of Microglial Cultures to Lipopolysaccharide
- 08:46Conclusion
Isolering primære microglia fra cellulær heterogenicitet i hjernen er vigtigt at undersøge deres rolle i både fysiologiske og patologiske tilstande. Denne protokol beskrives en mekanisk isolation og blandet cellekultur teknik, som tilvejebringer et højt udbytte og høj renhed, levedygtige primære mikrogliaceller celler til<em> In vitro</em> Studie-og downstream-applikationer.
Tags
Primary Microglia IsolationMixed Glial Cell CulturesMicroglia Role In Brain PhysiologyPhagocytic ActivityMicroglial ActivationNeurodegenerative DiseaseTraumatic Brain InjuryNervous System InfectionReactive Oxygen And Nitrogen SpeciesPro-inflammatory Chemokines And CytokinesCellular Heterogeneity Of The BrainNeuroprotective And Neurotoxic Functions Of MicrogliaIsolation Protocol For Primary Microglia
