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JoVE Journal Bioengineering
Dyrking av Human Neural stamceller i en 3-dimensjonal Self-montering Peptid Hydrogel
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From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
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Dyrking av Human Neural stamceller i en 3-dimensjonal Self-montering Peptid Hydrogel

Article DOI: 10.3791/3830-v • 11:02 min • January 11th, 2012
January 11th, 2012 •
1, 1, 1
1Albrecht-Kossel-Institute for Neuroregeneration, University of Rostock

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Her beskriver vi bruk av en selv-montering 3-dimensjonale stillas til kultur menneskelige nevrale stamceller. Vi presenterer en protokoll for å frigjøre cellene fra stillasene for å bli analysert senere for eksempel ved flowcytometri. Denne protokollen kan tilpasses andre celletyper å utføre detaljerte mechanistically studier.

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Bioteknologi PuraMatrix RADA16 3D-stillaset ReNcell VM menneskelige nevrale stamceller kvantifisering
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