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JoVE Journal
Biology

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Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
 

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Article DOI: 10.3791/3928-v 11:39 min September 21st, 2012
September 21st, 2012

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Summary

Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.

Tags

Unfixed Tissues Frozen Tissues Natural Mucin Distribution Mucins Glycoproteins Mucus Layer Host Innate Immunity Antimicrobial Peptides Cytokines Immunoglobulins Histological Methods Tissue Collection Staining Formalin Fixation Paraffin Embedding Tissue Freezing Cryo-protectant Media
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