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In vitro analyse van PDZ-afhankelijke CFTR Macromoleculaire signaalcomplexen
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In vitro analyse van PDZ-afhankelijke CFTR Macromoleculaire signaalcomplexen

Article DOI: 10.3791/4091-v • 10:05 min • August 13th, 2012
August 13th, 2012 •
1, 1, 1,2,3
1Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, 2Cardiovascular Research Institute, Wayne State University School of Medicine, 3Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

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Cystic fibrosis transmembrane conductance regulator (CFTR), een epitheliale chloride kanaal, is gemeld om te communiceren met verschillende eiwitten en belangrijke cellulaire processen te reguleren, onder wie de CFTR PDZ motief-gemedieerde interacties zijn goed gedocumenteerd. Dit protocol beschrijft methodes die we ontwikkeld tot een PDZ-afhankelijke CFTR macromoleculaire signaleren van complexe monteren

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Biochemie Moleculaire Biologie Scheikunde CFTR macromoleculaire complex eiwit interacties PDZ scaffold proteïne epitheelcellen cystic fibrosis
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