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Een High-inhoud Imaging Workflow om Grb2 signaalcomplexen Studie door Expression Klonen
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Biology
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A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
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Een High-inhoud Imaging Workflow om Grb2 signaalcomplexen Studie door Expression Klonen

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10,483 Views

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10:52 min

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October 30, 2012

DOI:

10.3791/4382-v

DOI:
10.3791/4382-v

•

10:52 min
October 30, 2012

•

1 Views
, , ,
1MRC LMCB,University College London, 2Center for Computational and Integrative Biology,Massachusetts General Hospital

Summary

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Een high-content screening methode voor de identificatie van nieuwe signalering bevoegde transmembraanreceptoren beschreven. Deze methode is ontvankelijk voor grote schaal automatisering en maakt voorspellingen<em> In vivo</em> Eiwitbinding en de subcellulaire lokalisatie van eiwitcomplexen in zoogdiercellen.

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Freeman, J., Kriston-Vizi, J., Seed, B., Ketteler, R. A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning. J. Vis. Exp. (68), e4382, doi:10.3791/4382 (2012).

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