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JoVE Journal Bioengineering
Bakteriell Detection & identifiering med elektrokemiska sensorer
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Bakteriell Detection & identifiering med elektrokemiska sensorer

Article DOI: 10.3791/4282-v • 09:30 min • April 23rd, 2013
April 23rd, 2013 •
1,2, 3, 2, 2,4,5
1Research Service, Veterans Affairs Greater Los Angeles Healthcare System, 2Department of Urology, The David Geffen School of Medicine, University of California, Los Angeles, 3GeneFluidics, 4Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, 5Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles

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Vi beskriver en elektrokemisk metod sensor analys för snabb bakteriell detektion och identifiering. Analysen omfattar en sensor array med funktionella fånga DNA oligonukleotidsonder för ribosom RNA (rRNA) artspecifika sekvenser. Sandwich hybridisering av mål-rRNA med infångningsproben och en pepparrotsperoxidas-länkad DNA oligonukleotid detektorsonden ger en mätbar amperometriskt ström.

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Bioteknik mikrobiologi genetik molekylärbiologi cellbiologi biokemi medicinsk teknik medicin immunologi bakterier elektrokemisk sensor ribosom-RNA rRNA 16S RNA DNA sond analys
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