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Verbeterd protocol voor Laser Microdissection der humane pancreatische eilandjes Van Chirurgische Specimens
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Verbeterd protocol voor Laser Microdissection der humane pancreatische eilandjes Van Chirurgische Specimens

Article DOI: 10.3791/50231-v • 09:44 min • January 6th, 2013
January 6th, 2013 •
1,2, 3, 1,2, 1, 2, 1,2, 2, 4, 5, 6, 7, 8, 9, 2, 10, 3, 1
1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis

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Laser microdissectie is een techniek die het herstel van de geselecteerde cellen uit minieme hoeveelheden parenchym mogelijk maakt. Hier beschrijven we een protocol voor die menselijke pancreatische eilandjes van chirurgische preparaten worden gebruikt voor transcriptoom studies. Ons protocol verbetert de intrinsieke autofluorescentie van menselijke beta-cellen, waardoor hun collectie.

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Geneeskunde Fysiologie Anatomie Biochemie Celbiologie moleculaire biologie immunologie chirurgie diabetes mellitus type 2 laser microdissectie dissectie menselijke beta-cellen intrinsieke autofluorescentie alvleesklier partiële resectie Diabetes type 2 transcriptoom studies RNA analyse islet
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