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Operation of a Benchtop Bioreactor
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Operation of a Benchtop Bioreactor
DOI:

12:54 min

September 12, 2013

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Chapters

  • 00:05Title
  • 01:40Fermentor Vessel Set-up
  • 02:31Fermentor Vessel Set-up: pH Probe Calibration
  • 03:49Fermentor Vessel Set-up: Continuation of Vessel Set-up and Calibration of pO2 Probe
  • 08:01Fermentor Vessel Set-up: Inoculation and Sampling
  • 10:03Results: A Typical Bioreactor Run
  • 12:07Conclusion

Summary

Automatic Translation

Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.

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