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Derribo Gene a gran escala en
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Derribo Gene a gran escala en

Article DOI: 10.3791/50693-v • 18:38 min • September 25th, 2013
September 25th, 2013 •
1, 2, 3
1Molecular and Cellular Biology Program, University of Massachusetts, Amherst, 2Neuroscience and Behavior Program, University of Massachusetts, Amherst, 3Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst

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Mientras dsRNA alimentación en C. elegans es una poderosa herramienta para evaluar la función de los genes, los protocolos actuales para pantallas de alimentación a gran escala resultan en eficiencias desmontables variables. Se describe un protocolo mejorado para realizar a gran escala pantallas de alimentación de RNAi que se traduce en caída altamente eficaz y reproducible de la expresión génica.

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Biología del Desarrollo número 79 Caenorhabditis elegans (C. elegans) Técnicas Gene Knockdown, La interferencia de ARN de doble cadena gen desmontables pantalla de la alimentación a gran escala
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