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Immunology and Infection

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JoVE Journal Immunology and Infection
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Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Article DOI: 10.3791/51177-v • 16:21 min • March 10th, 2014
March 10th, 2014 •
1,2, 1, 2
1Microbiology Unit, Department of Biochemistry, University of Oxford, 2Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford

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Summary
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Photoactivated localization microscopy (PALM) combined with single-molecule tracking allows direct observation and quantification of protein-DNA interactions in live Escherichia coli cells.

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Protein-DNA Interactions Live Bacterial Cells Photoactivated Single-molecule Tracking DNA Replication Transcription Repair Chromosome Organization DNA-binding Proteins In Vitro Experiments Detailed Models Function Of DNA-binding Proteins Complex Environment Of The Living Cell Quantifying DNA-repair Activities Escherichia Coli Cells Photoactivated Localization Microscopy (PALM) Single-molecule Tracking Individual DNA-binding Events Protein Activity Abundance Of Substrates Binding Sites Single-cell Level Sample Preparation Data Acquisition Data Analysis Procedures
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