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Journal / Immunology and Infection / Propagação de
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Immunology and Infection

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Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
Journal (Immunology and Infection)
Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
Journal (Immunology and Infection)
Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
 
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Propagação de

Article DOI: 10.3791/51953-v • 12:52 min • September 25th, 2014
September 25th, 2014 •
1, 1, 2, 1
1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS

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Aqui apresentamos um protocolo para propagar células Homalodisca vitripennis e HoCV-1 in vitro. Médio foi removido do HoCV-1 culturas positivas e RNA extraído a cada 24 horas para 168 horas. A capacidade de sobrevivência das células foi quantificada por coloração com azul de tripano. Partículas de vírus inteiros foram extraídos após a infecção. RNA extraído foi quantificada por qRT-PCR.

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Infecção Edição 91, Cultura celular a doença de videira de Pierce,
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