Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Purificação de preparações de vesículas…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Purificação de preparações de vesículas extracelulares de alto rendimento longe do vírus
Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
Journal (Immunology and Infection)
Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
Journal (Immunology and Infection)
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
 
Click here for the English version

Purificação de preparações de vesículas extracelulares de alto rendimento longe do vírus

Article DOI: 10.3791/59876-v • 07:15 min • September 12th, 2019
September 12th, 2019 •
*1, *1, 1, 1, 1,2, 3, 4, 5, 1
1Laboratory of Molecular Virology, School of Systems Biology, George Mason University, 2American Type Culture Collection (ATCC), 3ATCC Cell Systems, 4Ceres Nanosciences, Inc, 5Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine, Florida International University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Este protocolo isola vesículas extracelulares (EVs) longe de virions com alta eficiência e rendimento através da incorporação de precipitação EV, ultracentlee gradiente de densidade, e captura de partículas para permitir um fluxo de trabalho simplificado e uma redução de partida requisitos de volume, resultando em preparações reprodutíveis para uso em todas as pesquisas EV.

Tags

Imunologia e infecção vesículas extracelulares exosome vírus purificação ultracentlee gradiente de densidade precipitação nanopartículas EV-enriquecedora
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter