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JoVE Journal
Developmental Biology

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在小鸡视网膜有效的基因转移的原代细胞培养研究:
 
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在小鸡视网膜有效的基因转移的原代细胞培养研究:

Article DOI: 10.3791/52002-v 08:59 min November 2nd, 2015
November 2nd, 2015

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Summary

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鸡胚视网膜细胞培养物是宝贵的工具,用于感光生物学研究。我们已经开发了基于前OVO之前培养的视网膜质粒电高效的基因转移技术。这种技术显着地增加的转染效率比现有的协议,使遗传操作此系统是可行的。

Tags

发育生物学,第105,视网膜,感光器,原代细胞培养,电穿孔,质粒转染,小鸡
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