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JoVE Journal
Developmental Biology

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鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔
 
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鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔

Article DOI: 10.3791/53421
December 14th, 2015

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Summary December 14th, 2015

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小脑外部颗粒层是最大的过境扩增在显影脑的部位。在这里,我们提出了一个协议,以目标基因改造这个层扩散采用体外电击和小脑切片文化从胚胎第14天鸡胚的高峰。

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