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Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
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Chapters
- 00:05Title
- 01:26Seminiferous Tubule Preparation
- 04:41Peritubular Cell (PTC) Isolation
- 06:30Sertoli Cell Isolation
- 08:37Results: Representative PTC and Sertoli Cell Culture
- 09:31Conclusion
Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.
