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JoVE Journal Genetics
Isolering af Giant børstekromosomer Kromosomer fra Living Oocyter af frøer og salamandre
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Isolering af Giant børstekromosomer Kromosomer fra Living Oocyter af frøer og salamandre

Article DOI: 10.3791/54103-v • 10:07 min • December 5th, 2016
December 5th, 2016 •
1, 1
1Department of Embryology, Carnegie Institution for Science

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Vi præsenterer enkle teknikker til isolering gigantiske transkriptionelt aktive børstekromosomer kromosomer fra levende oocytter af frøer og salamandre. Vi beskriver, hvordan overholdelse af disse kromosomer "live" af fasekontrast eller differentiel interferens kontrast, og hvordan man kan løse dem for fluorescerende in situ hybridisering eller immunfluorescensfarvning.

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Genetik Germinal vesikel (GV) histon locus krop børstekromosom kromosom nuklear kuvert nuklear speckle kernelegeme oocyt transskription
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