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Journal / Biochemistry / Påvisning af pH-afhængige aktivitet af
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JoVE Journal Biochemistry
Påvisning af pH-afhængige aktivitet af
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Påvisning af pH-afhængige aktivitet af

Article DOI: 10.3791/54527-v • 08:32 min • October 23rd, 2016
October 23rd, 2016 •
1, 1,2, 1,2, 1
1Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 2Howard Hughes Medical Institute, University of Michigan

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Denne undersøgelse beskriver biofysiske, biokemiske og molekylære teknikker til at karakterisere chaperonaktivitet af Escherichia coli HdeB under sure pH-betingelser. Disse metoder er blevet anvendt med succes til andre syre-beskyttende chaperoner såsom HdeA og kan ændres til at arbejde for andre chaperoner og stresstilstande.

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Biokemi Molecular Chaperone Acid Stress Stress respons Protein Aggregation Protein Udfoldning Bakterier lysspredning Protein Genfoldning protein-protein interaktion Protein Kvalitetskontrol.
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