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Journal / Cancer Research / Modificeret Terminal Restriktion Fragment Analyse …
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Cancer Research

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JoVE Journal Cancer Research
Modificeret Terminal Restriktion Fragment Analyse til kvantificering af telomer længde ved brug af in-gel hybridisering
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Modificeret Terminal Restriktion Fragment Analyse til kvantificering af telomer længde ved brug af in-gel hybridisering

Article DOI: 10.3791/56001-v • 11:29 min • July 10th, 2017
July 10th, 2017 •
1,2, 2, 2,3, 2,4, 2,3
1Departments of Pathology and Infectious Diseases and Microbiology, University of Pittsburgh, 2University of Pittsburgh Cancer Institute, 3Department of Environmental and Occupational Health, University of Pittsburgh, 4Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences, University of Pittsburgh

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I denne artikel diskuteres en detaljeret protokol til kvantificering af telomerlængden ved hjælp af en modificeret terminalbegrænsningsfragmentanalyse, som giver hurtig og effektiv direkte måling af telomerlængde. Denne teknik kan anvendes på en række cellekilder til DNA til kvantificering af telomerlængden.

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Cancer Research udgave 125 Southern blot terminale restriktionsfragmenter leukocyttelomerlængde telomerlængde in-gel-hybridisering DNA-hybridisering
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