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Journal / Cancer Research / Modifisert Terminal Restriksjonsfragmentanalyse…
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JoVE Journal Cancer Research
Modifisert Terminal Restriksjonsfragmentanalyse for kvantifisering av telomer lengde ved bruk av in-gel hybridisering
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Modifisert Terminal Restriksjonsfragmentanalyse for kvantifisering av telomer lengde ved bruk av in-gel hybridisering

Article DOI: 10.3791/56001-v • 11:29 min • July 10th, 2017
July 10th, 2017 •
1,2, 2, 2,3, 2,4, 2,3
1Departments of Pathology and Infectious Diseases and Microbiology, University of Pittsburgh, 2University of Pittsburgh Cancer Institute, 3Department of Environmental and Occupational Health, University of Pittsburgh, 4Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences, University of Pittsburgh

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I denne artikkelen diskuteres en detaljert protokoll for kvantifisering av telomerlengden ved hjelp av en modifisert terminalrestriksjonsfragmentanalyse som gir rask og effektiv direkte måling av telomerlengden. Denne teknikken kan brukes på en rekke cellekilder til DNA for kvantifisering av telomerlengden.

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Cancer Research utgave 125 Southern blot terminal restriksjon fragmenter leukocyt telomer lengde telomer lengde in-gel hybridisering DNA hybridisering
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